Genotyping - Sequencing (GenSeq) Environmental Genomics
This platform provides the community with traditional capillary electrophoresis genetic analysis technologies: fragment analysis, microsatellites, AFLP genotyping and Sanger sequencing. It also provides automated extraction of genomic-grade nucleic acids in order to rapidly and reproducibly treat a large number of samples. NGS technologies are also available.
In addition to genotyping and sequencing by capillary electrophoresis, GenSeq offers cutting-edge technologies for the preparation of libraries for high-throughput sequencing: high-precision sonication and automatic collection of DNA fractions. A Biomek 4000 pipetting robot is available for sample handling and purification of PCR products or 96-well sequencing. A robot has been installed (2014) allowing magnetic bead extraction of 96 samples in less than one hour.
- KingFisher Flex DNA or RNA automatic extraction robot
- MiSeq Illumina sequencer
- ABI 3130XL 16 capillary and ABI 3500XL 24 capillary sequencers
- Biomek 4000 pipetting robot
- Covaris S220 sonicator
- Pippin Prep automatic DNA collector
- 2 Eppendorf thermocyclers, one with a temperature gradient
- Nanodrop 8000 Spectrophotometer
- Qubit Fluorimeter
For genotyping, the platform only performs capillary electrophoresis, potentially preceded by a reassembly of samples on a 384-well plate. The schedule of passages is available online.
The facility can prepare Sanger sequencing reaction products and purification, or simply process users’ reaction products.