Cyto – Evolutionary Genomics Environmental Genomics
This platform is shared between two, complementary sites. It is dedicated to evolutionary biology analyses based on chromosomes, from classic karyotyping to the latest in situ hybridization techniques. It provides the tools needed by cytogenetic projects that study the architecture and/ or function of the genome, chromatin, chromosomes and genes.
Evolutionary cytogenetics studies the architecture and functioning of the genome at the level of genes, chromatin and chromosomes. It primarily targets the organisation of the chromosome to discover the evolutionary processes associated with changes in chromosome structure and the consequence of these changes in terms of adaptation and speciation.
The platform is located at two laboratories. The first, on the Triolet Campus at the University of Montpellier (ISEM) hosts imaging equipment and a collection of cryopreserved cells. The second is at the Baillarguet Campus (CBGP), which is equipped with imaging equipment and a cell culture unit.
A wide range of techniques is available, from conventional cytogenetic techniques (karyotyping, G, C and NOR-banding) to molecular cytogenetic techniques based on in situ hybridization and fluorescence microscopy (chromosomal painting, ZOO FISH, BAC FISH, PRINS, sperm FISH, etc.), and functional genomics techniques (epigenetic analysis, gene expression) in collaboration with other environmental genomics platforms.
- Three Zeiss Axiophot fluorescence microscopes and image analysis systems (Genus)
- An inverted microscope
- Two PCR units with blocks for microscopic slides (for PRINS)
- Two extractor hoods
- A laminar flow hood
- A CO2 thermostated oven for cell culture
- A system for cryopreservation of cell lines
- Two hot plates
- 500 karyotypes (wild rodents, fish, mosquitoes);
- start-up, maintenance and cryopreservation of cell lines;
- 500 chromosomal localizations of genes or chromosomal fragments by in situ hybridization (Zoo FISH, BAC FISH) or PRINS.
Users: IFR units (ISEM, CBGP, MIVEGEC), external (IGH, University of Toulouse.), students (Diploma, Masters, PhD), post-docs, foreign partners (UCAD, Senegal; UAM, Niger, etc.)